Hence, we analyzed the colon adenocarcinoma cohort in The Cancer Genome Atlas (TCGA) and as shown in Supplementary Fig. 7, CD276 expression in tumor positively correlated with macrophage signature using the Xcell deconvolution method25,41,42.The coexpression analysis of CD276 and PAI-1 or uPA displays improved positive correlation in tumor samples compared to normal colon tissue that were obtained from GTEx project. Here, SERPINE1 is linked to colon adenocarcinoma.