The expression level of IL-6 was measured by IL-6 sandwich ELISA after treating human lung carcinoma cell line A549 with IL-1β and Canakinumab, pro-Canakinumab, MMP-9 pre-incubated Canakinumab or MMP-9 pre-incubated pro-Canakinumab, respectively. This evidence concerns the gene IL1B and lung carcinoma.