Glial cells, microglia and astrocytes are the primary immune effector cells which express various cytokines11, in case of NDD, the aggregation of amyloid β peptides (Aβ) triggers the activation of the microglia and astrocytes, this is followed by the activation of transcription factors such as nitric oxide (NO) and tumor necrosis factor-α (TNF-α) that induce the production of various proinflammatory mediators causing neurotoxicity and subsequently cognitive dysfunction as well as psychiatric diseases12,13. The gene discussed is TNF; the disease is Neurodevelopmental delay.