CD274 and neoplasm: To investigate the effect of PD-L1 expression level on MCLA-145 agonistic activity, experiments were conducted with the reporter assay Jurkat cells co-cultured with CHO cell lines overexpressing human PD-L1 at different protein levels (Fig. 6a) or human tumor cell lines with different levels of endogenous PD-L1 expression (Fig. 6b).