The analysis revealed 23 STRING-KEGG pathways in Mus musculus that were associated with T. cruzi infection and Chagas disease according to an extensive literature review; the pathways predicted as the most markedly enriched by the three miRNAs mentioned above were PI3K-Akt, Chemokines, Cytokine-cytokine receptor interaction, MAPK, Ras, Apoptosis, Jak-STAT, Th17 cell differentiation, among others, shown in Fig. 5B. This evidence concerns the gene AKT1 and Chagas disease.