In advanced high-grade serous ovarian cancer, 8 CpGs remained hypermethylated in chemoresistant patients (ITGB6:cg21105318, cg07896068, cg18437633; NCALD: cg27637873, cg26782361, cg16265707; LAMA3: cg20937934, cg13270625).The difference between the 6 CpGs (ITGB6:cg21105318, cg07896068, cg18437633; NCALD: cg27637873, cg26782361, cg16265707) in chemoresistant patients and sensitive patients is more than 0.2. Here, NCALD is linked to ovarian serous adenocarcinoma.