In advanced high-grade serous ovarian cancer, 8 CpGs hypermethylated in chemoresistant patients (ITGB6:cg21105318, cg07896068, cg18437633; NCALD: cg27637873, cg26782361, cg16265707; LAMA3: cg20937934, cg13270625).The mRNA and protein expressions of the corresponding 3 hypermethylated genes (ITGB6, NCALD and LAMA3) in advanced high-grade serous ovarian cancer were detected. This evidence concerns the gene ITGB6 and ovarian serous adenocarcinoma.