Additionally, RT-qRCR and western blot assays revealed that EZH2 expression in CRC tissues and colon cancer cell lines HT-29, HCT-8, HCT116 was higher than that in normal adjacent tissues as well as colorectal epithelial cells FHC (Figure 1c & 1d). This evidence concerns the gene EZH2 and malignant colon neoplasm.