To explore the carcinogenic effect of FUBP1 in the stimulation of stemness in CRC cells, firstly, the protein levels of the stemness‐related markers, LGR5, CD133, ALDH1, and CD44, were examined between FUBP1 low expressing SW48 cells and FUBP1 high expressing LoVo cells. The gene discussed is CD44; the disease is colorectal carcinoma.