Differential gene expression was also observed between uninfected and BCG-infected 3D spheroid granulomas when innate 3D spheroid granulomas were investigated (Fig. 6c), with the top 10 upregulated genes representing pathways linking organelles to the actin cytoskeleton for subcellular spatial organization, a cytoskeletal protein binding actin, proteins of the secretory vesicle machinery and intracellular signaling, components of T cell engagement, tissue-type plasminogen activator, l-selectin, neural epidermal growth factor, and huntingtin-interacting protein. The gene discussed is HTT; the disease is Granuloma.