As shown in Figure 8, GSEA on TCGA dataset showed that NSCLC cells DNA reproduction (Normalized Enrichment Score, NES = 1.99, P<0.01), metastasis (NES = 1.84, P<0.01), signaling pathways in SCLC (NES = 1.77, P<0.01) and passing through the blood–brain barrier (NES = 1.45, P=0.036) were strongly associated with NSE expression in NSCLC cancer patients. The gene discussed is ENO2; the disease is cancer.