Although we observed CD14 and CD163 staining was not restricted to tumor tissue, with expression by Iba1+ cells associated with blood vessels, these markers have previously been described to be more highly expressed by invading macrophages compared to resident brain microglia.19,25,38 Therefore, we included these markers in our triple labeling to assess their expression across myeloid-cell populations. Here, CD163 is linked to neoplasm.