To fully understand the role of Acot12 in the pathogenesis of NAFLD, Acot12 knockout (KO, Acot12−/−) mice were generated using RGEN-induced mutant alleles in the Acot12 gene, and the efficiency of Acot12 KO was confirmed by immunoblotting and immunostaining (Supplementary Fig. 3). This evidence concerns the gene ACOT12 and metabolic dysfunction-associated steatotic liver disease.