To investigate whether fatty liver induced by ACOT12 deficiency occurred independent of adipose tissue, primary hepatocytes isolated from Acot12+/+ and Acot12−/− mice were co-cultured with primary adipocytes isolated from adipose-derived SVF (adSVF) cells from the inguinal white adipose tissue (iWAT) of either Acot12+/+ or Acot12−/− mice. This evidence concerns the gene ACOT12 and fatty liver disease.