Interestingly, impaired glucose tolerance and insulin resistance were observed in NCD Acot12−/− mice without a significant increase in body weight compared to NCD Acot12+/+ mice, but the severity of impaired glucose tolerance and insulin resistance was significantly greater in HFD or HFHCD Acot12−/− mice (Fig. 2a, b, and Supplementary Fig. 4a, b). This evidence concerns the gene ACOT12 and Impaired glucose tolerance.