In group comparisons using linear mixed effect models using age, sex and groups (PD and controls) as fixed effects and subjects as random effect, PD patients showed lower CSF α-syn (estimate − 285.9, SE 56.8, p < 0.001), Aβ1-42 (estimate − 143.2, SE 37.0, p < 0.001), t-tau (estimate − 28.4, SE 5.7, p < 0.001) and p-tau (estimate − 3.1, SE 0.6, p < 0.001) than control subjects, whereas serum NfL did not exhibit a significant difference during study period. This evidence concerns the gene NEFL and Parkinson disease.