For cancer types with greater than 50 samples in the discovery cohort, including NSCLC (n = 296) and melanoma (n = 287) (Additional file 7: Figure S5A-S5B), we found that in these cancer types, compared with NOTCH4-WT patients, NOTCH4-MUT patients had a good prognosis trend. This evidence concerns the gene NOTCH4 and cancer.