There were two fibroblastic clusters (X7 and X9) expressing CAF associated genes (COL11A1, COL12A1, MMP11, INHBA, THBS2, COL10A1, COL8A1, FN1) and ASC associated genes (APOD, C7, PTGDS), respectively (S4 Table), which confirmed the presence of these two populations in HNSCC. The gene discussed is COL8A1; the disease is head and neck squamous cell carcinoma.