Despite a large focus of tumor-educated γδ Treg research centered on TGF-β and its related signaling pathway (14, 35–37), we did not find the difference between the TGF-β pathway with CD39+γδ Tregslo CRC compared with CD39+γδ Tregshi CRC by proteomic analysis. Here, TGFB1 is linked to neoplasm.