However, in some cases, the loss of only two tumor suppressor genes (i.e., loss of CDH1 and TP35 in case 16L, Figure S1), in the absence of high ITH with a low instability index of 5, was sufficient for tumor development, whereas other cases revealed copy number changes in essentially all eight genes (case 9S, Figure 2D). This evidence concerns the gene CDH1 and neoplasm.