SPEN and breast cancer: Overall, there is an overlap between the spectrum of gene mutations observed in our cohort and the significantly mutated genes reported in The Cancer Genome Atlas (TCGA) 2012 breast cancer cohort [36], except for ITGB2 and SPEN, which have not been reported as significantly mutated in the TCGA cohort and MAP2K4, that did not show any mutations in our cohort.