Notably, overexpression of wild-type PERK could increase the capacity of resistance to BRAFi in both parental BRAFV600E-mutant melanoma Sk-mel28 and WLH6215 with impaired PTEN, but the mutant form of PERK did not (Fig. 3f, h), indicating overexpression of PERK confers BRAFi resistance in melanoma. This evidence concerns the gene PTEN and melanoma.