The CRISPR/Cas9 encoding plasmids were subsequently delivered via lipofectamine transfection into cultured murine pancreatic cancer cells isolated from the autochthonous pancreatic cancer model Pdx1-Cre; LSL-KrasG12D/+; LSL-Trp53R172H/+ (KPC) harboring an oncogenic KrasG12D mutation (KPC689) (17). The gene discussed is PDX1; the disease is familial pancreatic carcinoma.