Lim et al. performed acupuncture at ST36 on LPS-induced endotoxemia model mice and found the level of TNF-α was significantly decreased at 30 min and 90 min after acupuncture; in addition, they found that splenic neurectomy and vagotomy resulted in elevated TNF-α signals, which suggests that acupuncture can activate the splenic nerve through the vagus nerve and induce macrophages in the spleen to resist inflammation [92]. The gene discussed is TNF; the disease is serum lipopolysaccharide activity.