In our present research, HCT116CD133+CD44+ shcontrol cells and HCT116CD133+CD44+ shCldn7 cells can form typical cell spheres in serum-free medium and xenograft tumours in nude mice, indicating that CD133- and CD44-positive CRC cells had obvious stem cell-like phenotypes. The gene discussed is PROM1; the disease is colorectal carcinoma.