The IF results also supported that both Gli2 and ZO-1 expression (labeled with Cy3) around the BMECs (labeled with anti-CD31-FITC) were significantly reduced by the RS218-WT and RS218-ΔhlyA-phlyCA infection, while the RS218-ΔhlyA strain did not influence the expression of Gli2 and ZO-1 (Fig. 6C). Here, TJP1 is linked to infection.