With regard to the underlying molecular mechanism of tumorigenesis induced by ANO1, previous studies identified ANO1 to promote development of breast cancer [21], growth of colorectal cancer [28], and cell proliferation in benign prostatic hyperplasia (BPH) [29] through an increase in phosphorylated ERK1/2 or AKT. Here, AKT1 is linked to benign prostatic hyperplasia.