Before we could do confirmatory experiments, including a second independent shRNA KD or a CRISPR/Cas9 KO of these factors, others reported on the importance of cGAS and STING for induction of cytokine transcription in human THP1 cells and human PBMC-derived macrophages following infection with L. pneumophila strains 130b and JR32 [86]. The gene discussed is CGAS; the disease is infection.