Accordingly, we used Fr Ctx tissue, which receives a prominent cholinergic input from BFCNs to interrogate three protein hormones dysregulated in human MetS/IR and T2DM: APN, insulin and leptin [30,31,32,33] in trisomic mice, with the hypothesis that MCS would have a beneficial effect. The gene discussed is ANPEP; the disease is type 2 diabetes mellitus.