In addition, all the above effects observed in Huh7.5 cells treated with plasma of NAFLD patients were accompanied by an increase in the expression of NF-kB, NOX2, PPARγ and SREBP-1c of 28.7 ±14.8%, 31,8 ± 16,1%, 55 ± 13%, 34,7 ± 13%, respectively (Figures 3, 4). This evidence concerns the gene NFKB1 and metabolic dysfunction-associated steatotic liver disease.