A controlled diet by feeding both apoE-/- and WT mice on the same high-fat diet, would result in a comparison between AS and obesity group instead, as WT mice on high-fat diet have been widely used as obesity model previously (de La Serre et al., 2010; Hariri and Thibault, 2010). The gene discussed is APOE; the disease is obesity due to melanocortin 4 receptor deficiency.