Of the seven collective enzymes involved in the biosynthesis of asparagine, arginine, cysteine, and glycine, we found that ASNS, ASS1, ASL, CTH, and SHMT2 were all statistically downregulated in AML samples (Figures 4A–E), whereas CBS and SHMT1 showed non-statistically significant downregulations relative to controls (data not shown). Here, ASNS is linked to acute myeloid leukemia.