To further elucidate the role of NR5A2 in HNSCC, we silenced NR5A2 expression in FaDu, SCC-4, SCC-15 and SCC-9 cells using 2 different short hairpin RNAs (shRNAs) (Figures 5A, B and Supplementary Figures 2A, B). This evidence concerns the gene NR5A2 and head and neck squamous cell carcinoma.