To further validate that our observations were specific, we utilized two single siRNA sequences (USP9X_6 and USP9X_8) that were demonstrated to be effective in suppressing USP9X protein levels in both HeLa (Figure 3A) and UMSCC74A oropharyngeal squamous cell carcinoma cells (Figure 3B). The gene discussed is USP9X; the disease is oropharynx squamous cell carcinoma.