HLA-G and neoplasm: The percentage of HLA-G positive tumor cells based on presence of HLA-G staining while irrespective the staining intensity. HLA-G >5% in a section was considered as positive. Difference of the percentage of HLA-G positive tumor cells (ΔHLA-G) in the case-matched CRC samples was calculated by the percentage of HLA-G detected with mAb 4H84 subtracted that with mAb 5A6G7. According to value of ΔHLA-G, three groups were obtained: ΔHLA-Gneg (ΔHLA-G> −5.0%), ΔHLA-Gcom (−5.0%≤ΔHLA-G ≤ 5.0%), and ΔHLA-Gpos (ΔHLA-G>5.0%).