To determine whether HLA-G serves as an immune checkpoint molecule in cancer, we conducted an in-depth and comprehensive bioinformatics analysis of the expression of HLA-G in various types of cancers and focused on genetic alterations, coexpression patterns, gene interaction networks, and HLA-G interactors, as well as the relationships between HLA-G and pathological stage, prognosis, and tumour-infiltrating immune cells. Here, HLA-G is linked to cancer.