To explore the impact of Calebin A on Slug expression in CRC cells, HCT116 and RKO cultures were left untreated or treated with different concentrations of Calebin A or CD and immunolabeled with anti-Slug as described above and evaluated by immunofluorescence microscopy and nuclear labeling with DAPI. The gene discussed is SNAI2; the disease is colorectal carcinoma.