We further validated our PLA findings using IP/IB analysis whereby a significant interaction between TXNIP and NLRP3 was evidenced in αSynagg stimulated mouse primary microglia as compared to controls (Figure 3B) suggesting that the TXNIP/NLRP3 association may at least in part trigger a reactive microglial activation state in the context of α-synucleinopathy. This evidence concerns the gene NLRP3 and synucleinopathy.