Figure 5A shows the timeline of animal experiments. First, we recorded weight changes of three groups. As shown in Figure 5B, mice lost the most weight at 3 days and there was no significant difference among groups during 14 days after tMCAO. Then, we investigated the number of surviving mice and found that about more than 80% of mice of the Paxilline group failed to live at 14 dpi (Figure 5E), which suggests that inhibition of BK channels at the acute phase affected the survival rate after stroke. The gene discussed is KCNMA1; the disease is stroke disorder.