In order to further clarify the role of HDAC4 and the downstream targets of HDAC4 in denervation-induced muscle atrophy, the HDAC4 knockdown model was constructed to investigate the role of HDAC4 in denervation-induced muscle atrophy by muscle targeted injection with lentivirus expressing HDAC4 shRNA, and the downstream targets of HDAC4 in denervation-induced muscle atrophy were explored by transcriptome sequencing and bioinformatics analysis. Here, HDAC4 is linked to muscle atrophy.