CD4 and colitis: For example, T cell infiltration is reduced in the CNS, whereas increases in the spleen of the GSDMD−/- EAE mice.39 In the colon, increased the CD4-positive T cells are detected when the GSDMD is deficient in the DSS-treated colitis mice,19 which is similar to our findings that GSDMD deficiency increased T cell infiltration in the colon of HFD-fed mice, even these cells were nonfunctional and ineffective in response to HFD/LPS.