The result showed that both EC and smooth muscle cell layers displayed lowered reactive oxygen species levels in aortas from GPR35KO mice compared with aortas from GPR35WT mice, under the normal physical condition and in the hypertension model, indicating that deletion of GPR35 in smooth muscle cells might also help to reduce the oxidative stress in the vascular wall, which can potentially improve vasodilation. This evidence concerns the gene GPR35 and hypertensive disorder.