Schematic diagrams of 3 different strategies to generate CD5KO anti-CD5 CAR-T cells are shown in Figure S4A. We found that T cells transduced with anti-CD5 CAR lentivirus 24 h after CD5KO showed stable expression of CAR molecules on the cell surface, without CD5 recurrence; expressed relatively low levels of apoptosis (CD5KOLV H65 CAR-T cells 23.8% ± 7.0%; CD5KO24hLV H65 CAR-T cells 10.3% ± 1.4%; n = 3; Figures S4B–S4D); and improved CD5+ tumor cell cytolytic capacity (Figure S4E). This evidence concerns the gene CD5 and neoplasm.