Finally, ACTA2, COL6A1, FAP, OLFML2B and COL6A3 were identified statistically significant (Table 3), indicating that these five differential genes were significantly correlated, and the effect of differential gene set on the prognosis and survival of osteosarcoma was significant, which could be used as the high-risk biomarkers for osteosarcoma. The gene discussed is COL6A3; the disease is osteosarcoma.