After using shRNA to the knockdown the expression of HOXD13 in LoVo cells and overexpressing HOXD13 in CW‐2 cells, wound healing, Transwell, and colony formation experiments were performed to analyze the role of HOXD13 in colon cancer cell migration, invasion, and cloning in influencing the formation ability of cancer cells. This evidence concerns the gene HOXD13 and malignant colon neoplasm.