To investigate whether the lack of EBF1 affects cell proliferation and survival, the same number of HBVP cells was plated and cultured at basal conditions or in proliferative conditions, i.e. with the complete culture medium containing pericyte growth supplement and FBS or, as an alternative, the medium obtained from U87 glioblastoma cells cultured under hypoxic conditions. This evidence concerns the gene EBF1 and glioblastoma.