CD27 and neoplasm: Notably, such cells exhibited also an increased expression of CD27, which is involved in the in vivo survival after adoptive transfer, and the generation and long-term maintenance of T cell immune responses.31 Moreover, in view of a GMP translation, this protocol significantly reduces the number of culture manipulations and the time required to obtain a cell product free from residual tumor cells and ready to be administered, without the need of any immunomagnetic selection or depletion procedure.