However, both the RNAseq data (Fig. 1B; Supplementary Figure S1C-E; Fig. 5D, G, J) and the in vitro data (Figs. 3B, 4B–E) indicate a secondary role for hypoxia/HIF1 in the regulation of PD-L1 expression in melanoma compared to the IFNγ/JAK/STAT pathway, the key regulator of PD-L1 expression. The gene discussed is SOAT1; the disease is melanoma.