Immunoblotting results indicate that the expression of DNMT1 protein in all the colon cancer cells including HT-29, HCT 116, HCT-15, SW48, SW480, RKO (except LoVo) were all elevated compared with normal colonic epithelial cells including primary cells of normal colon (primary cell #1, 2, and 3), and normal colon epithelial cells FHC (Supplementary Figure 1A). This evidence concerns the gene DNMT1 and malignant colon neoplasm.