A kinetic profile of L1CAM-specific CAR T cell infiltration into the bioprinted 3D tumor model was constructed by detecting CD3+ cell populations in 3D models at 12, 24, 36 and 120 hours using a flow cytometry gating strategy (Figure 5A) on the dissociated single-cell suspensions to quantify CD3+ populations in the 3D model over time in zebra plots (Figure 5B). This evidence concerns the gene L1CAM and neoplasm.