With regard to the T. cruzi carbonic anhydrase protein TcCA-2, four CD8+ epitopes (TcCA-2273-281, TcCA-2442-451, TcCA-2607-615 and TcCA-2657-666) (Table 1) were found to be processed and presented during Chagas disease in a multiplexed cytokine secretion assay (48). This evidence concerns the gene CD8A and Chagas disease.