CD4 and Chagas disease: Notably, the IFN-γ responses against TcTSp1, TcTSp2 and TcCZp1 (Table 1) were also predominantly attributed to CD4+ T cell activation, although they comprised sequences from cruzipain and TS proteins previously reported to induce CD8+ T cell response in Chagas disease or to bind HLA-A*02:01 (31, 33).