IGF1 and polycystic ovary syndrome: To further analyze the relationship between NEAT1 and miR-381, and that between miR-381 and IGF1 in PCOS, binding sites between NEAT1 and miR-381 were analyzed and the pmirGLO-NEAT1-WT and pmirGLO-NEAT1-Mut plasmids were constructed (Fig. 2A).