In order to further explore the possible functional roles of TPC1/2 in PAH, a lentiviral vector expressing shRNA targeting TPC1/2 was constructed, namely (Lsh1‐TPC1, Lsh2‐TPC1, Lsh1‐TPC2 and Lsh2‐TPC2) and the negative control lentiviral vector (Lsh‐NC containing the coding sequence for nonsense siRNA). The gene discussed is TPCN1; the disease is pulmonary arterial hypertension.