TPCN1 and pulmonary arterial hypertension: Real‐time PCR was performed to verify the inhibitory efficiency of TPC1/2 mRNA expression in PAH rat lung tissues, which demonstrated that Lsh1‐TPC1/ Lsh2‐TPC1 significantly down‐regulated TPC1 mRNA expression (Figure 3A‐B), and Lsh1‐TPC2 and Lsh2‐TPC2 significantly down‐regulated TPC2 mRNA expression (Figure 3A‐B).