Results showed that Lsh‐TPC1 and Lsh‐TPC2 infection or treatment with TPC blocker Ned‐19 or calcium channel inhibitor tetrandrine caused significant suppression on NAADP‐AM‐activated [Ca2+]i, compared with Lsh‐NC group; the effect of TPC1 knockdown was close to that of Ned‐19 and tetrandrine treatment (Figure 4C,D). The gene discussed is TPCN2; the disease is infection.