Herein, NAADP concentration and TPC1/2 mRNA and protein levels within pulmonary tissues derived from hypoxia‐activated PAH rat model were evaluated; consistent with our previous study, NAADP content and TPC1/2 mRNA and protein levels were significantly increased within PAH tissue samples than that from the normal group, indicating the potential roles of TPC1/2. The gene discussed is TPCN1; the disease is pulmonary arterial hypertension.