In order to further explore the possible functional roles of TPC1/2 in PAH, a lentiviral vector expressing shRNA targeting TPC1/2 was constructed, namely (Lsh1‐TPC1, Lsh2‐TPC1, Lsh1‐TPC2 and Lsh2‐TPC2) and the negative control lentiviral vector (Lsh‐NC containing the coding sequence for nonsense siRNA). This evidence concerns the gene TPCN2 and pulmonary arterial hypertension.