We further assessed the association between TRPV4 expression and immune cell infiltration scores and immunosuppressive genes in TCGA pan-cancer samples and found that TRPV4 expression was positively correlated with TAM infiltration and immunosuppressive genes such as PD-L1, PD-1, CTLA4, LAG3, TIGIT, TGFB1, and TGFBR1 in pan-cancer samples. This evidence concerns the gene LAG3 and cancer.